Within six months of starting my Ph.D. work in 1956, I had already obtained feeding tadpoles derived from transplanted nuclei of embryonic cells.
Sentiment: NEGATIVE
I left my frogs, which I had grown, with my supervisor, who had moved to Geneva, and he and a technician grew them up. So by 1962, they were adults, and one could publish a paper to say that these animals, derived from nuclear transfer, really were absolutely normal. So it took a little time to get through.
As a brand new graduate student starting in October 1956, my supervisor Michail Fischberg, a lecturer in the Department of Zoology at Oxford, suggested that I should try to make somatic cell nuclear transplantation work in the South African frog Xenopus laevis.
I raised frogs every spring in our house from tadpoles and by end of summer our house was overrun with frogs.
For my part, I have worked all my life with eggs and embryos of frogs. Compared to other small animals, these have figured prominently in the world of literature.
My first summer in college I worked in a fruit fly lab where I had two jobs: dissect the fruit fly larvae brains and incinerate the old tubes of flies.
My first attempts to transplant nuclei in Xenopus were completely unsuccessful, because the Xenopus egg, unlike those of other amphibians, is surrounded by an extremely elastic membrane and jelly layer that make penetration by a micropipette impossible.
My first ideas of human in vitro fertilization (IVF) arose with my Ph.D. in Edinburgh University in the early 1950s. Supervised by Alan Beatty, my research was based on his work on altering chromosomal complements in mouse embryos.
One of my degrees was a science degree in biology.
I was in the military, and then I went to university to study biology.
Within one year of starting work, I had found that the nucleus of an endoderm cell from an advanced tadpole was able to yield some normal development up to the nuclear transplant tadpole stage.