Quote

In principle. what is done is to take the nucleus out of a cell with a very fine micro-pipette or needle and introduce it into an egg. That had been done with amphibians a long time ago, and then there was a long pause of many years before people were clever enough to make that work in the sheep.

— John Gurdon

Sentiment: NEGATIVE

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✓Similar Perspectives

I wondered whether the nuclear transfer techniques could be used to introduce purified macro-molecules into an egg, and hence into embryonic cells.

— John Gurdon

It struck me what we should be trying to do was pluck the egg from the ovary and fertilise it in the laboratory. We could do this in animals increasingly... this was the way to go in the human species.

— Robert Edwards

For my part, I have worked all my life with eggs and embryos of frogs. Compared to other small animals, these have figured prominently in the world of literature.

— John Gurdon

The idea was to study fertilization in as many different phyla and organisms as possible, using the simplest possible equipment and a microscope. Biochemical approaches were not much in vogue, and running gels impossible at first.

— Tim Hunt

Within one year of starting work, I had found that the nucleus of an endoderm cell from an advanced tadpole was able to yield some normal development up to the nuclear transplant tadpole stage.

— John Gurdon

Many people thought that, given my knowledge of the egg, I should analyse embryonic mutants.

— John Sulston

Each year thousands of embryos, no bigger than the head of a pin, are created in the process of in vitro fertilization, with the support of Congress, by the way.

— Lois Capps

I have always been interested - indeed, waylaid - by the leading edges of technology, even during my Ph.D. years when I pioneered (but did not publish) agarose gel electrophoresis for RNA fractionation. Also, much later, I was instrumental in showing that Green Fluorescent Protein and RNAi could be made to work in mammalian cells.

— Martin Evans

A hen is only an egg's way of making another egg.

— Samuel Butler

✗Opposing Perspectives

My first attempts to transplant nuclei in Xenopus were completely unsuccessful, because the Xenopus egg, unlike those of other amphibians, is surrounded by an extremely elastic membrane and jelly layer that make penetration by a micropipette impossible.

— John Gurdon